During most of the Holocene, men and Przewalski horses sustained a predator-prey relationship, and men gradually intruded, fragmented and degraded the grasslands where these animals lived. Takhis were eventually dislodged from the Mongolian and Chinese steppes though the details of this lengthy, complex and unmonitored process remain largely unknown. What is clear is that anthropogenic and climatic pressure became unbearable to the horses during the first half of the 20th century, depleteting the species below recovery level.
Their last known territory prior to extinction, i.e. south-eastern Mongolia, only represented a small fraction of their much broader historic range. Yet, even there, the horses were not at rest from human presence and activities and had to retreat to areas of the Gobi Desert, which provide poorer and scarcer vegetation and water holes year round. The horses were ill-adapted to this harsher environment and their situation was further aggravated by the occurrence of unusually rough climatic events around this time and by poaching, in violation of the horse hunting ban of 1926. The species’ resilience quickly eroded as bands became smaller and sparser. Large groups were still commonly encountered in the early 1940’s, but by the end of the decade and during the next one fewer individuals were occasionally sighted in the region of Tachijn-Shar-Nuru (meaning the “yellow mountain of the wild horse”), south of the Mongolian Altai, on the border with Xinjiang (see map). The end was near.
The Mongolian scientist N. Dovchin was the last witness to provide an official report of a wild living Takhi in 1969: he spotted a lone stallion near gun-Tamga, a natural spring situated in the same area as the previous sightings (see map). All attempts to locate Przewalski horses in this area and other parts of their known 20th century Mongolian and Chinese range were unsuccessful after this date, which became the official year of the species’ extinction in the wild.
REFERENCES –  Przewalski’s Horse: The History and Biology of an Endangered Species, by Lee Boyd, Katherine A. Houpt, 1994, pp 15-16.  Przewalski’s Horse: The History and Biology of an Endangered Species, by Lee Boyd, Katherine A. Houpt, 1994, pp 15-16.  Status and Action Plan for the Przewalski’s Horse, S. Wakefield et al., in Equids: Zebras, Asses, and Horses, Edited by Patricia Des Roses Moehlman, IUCN/SSC Equid Specialist Group IUCN, 2002, p. 83.